M. Nurul Islam
Coordinator - Core Campus Mass Spectrometry Facility
Phone: 605-688-5151
Department of Chemistry and Biochemistry
Avera Health and Science Center, Room 247
Box 2202
South Dakota State University
Brookings, SD 57007
Core Campus Mass Spectrometry Facility at SDSU
This facility is located in the Avera Health and Science Center as part of the Department of Chemistry and Biochemistry on the laboratory and research side of the building. The equipment is available to university staff and students as well as to the general public for a fee. In order to use the equipment, please contact Brian Logue. He will let you know about availability as well as answer any specific questions.
Rates
| Instrument/Category | SDSU | Academic-non SDSU | Industry |
|---|---|---|---|
| QTRAP | $20/hr. | $40/hr. | $80/hr. |
| MALDI-TOF | $30/hr. | $80/hr. | $160/hr. |
| LTQ | $15/hr. | $30/hr. | $60/hr. |
| *MS Facility Personnel Time | $100/hr. | $150/hr. | $300/hr. |
*MS Facility Personnel Time encompasses requested activities specific to individual users, including face-to-face training, mobile phase preparation, column preparation, sequence setup, sample preparation, method development consultation and other user-requested services which require MS facility personnel time. This does not include troubleshooting, preventative maintenance, etc.
Equipment
Specifications
- Mass Range: m/z 5-1,250
- Resolution (m/Δm): 2,000
- Accuracy: 1 ppm
- Scan Speed: 20,000 Da/s
- Polarity switching in 50 ms
- Ionization Source: Electrospray Ionization
- Mass Analyzer: Triple Quadrupole
Common Applications
- Fast LC/MS
- Metabolite Identification
- Detection and confirmation of low level pesticides
- Protein/peptide quantitation for biomarker verification and validation
- Ultra-fast and selective MRM scans mean you can target and quantify thousands of compounds in a single analysis
Specifications
- Mass Range: m/z 500-1,000,000
- Resolution (m/Δm): 5,000 (linear mode), 20,000 (reflectron mode)
- Accuracy: 200 ppm (linear mode), 10 ppm (reflectron mode)
- 337 nm Laser
- Ionization Source: Matrix-assisted laser desorption ionization (MALDI)
- Mass Analyzer: Time of flight (TOF)
Common Applications
- Characterization of biopolymers (proteins, protein digests and oligonucleotides)
- Organometallic complexes
- Some synthetic polymers
- Molecules that photoionize upon radiation by 337 nm laser
- Labile molecules
Specifications
- Mass Range: m/z 150-2,000
- Resolution (m/Δm): 4,000
- Accuracy: 100 ppm
- Ionization Source: Electrospray Ionization (ESI)
- Mass Analyzer: Linear Ion Trap
Common Applications
- High-speed LCMS
- Proteomics/metabolomics
- Structural elucidation
- Protein identification and quantitative differential expression analysis
- Biomarker studies and post-translation modification identification
- Metabolite identification and quantification
Advisory Committee
Douglas Raynie
Professor and Department Head Emeritus of Chemistry and Biochemistry
Department of Chemistry, Biochemistry and Physics
Brian Logue
Department Head/Professor
Department of Chemistry, Biochemistry and Physics
College of Natural Sciences
Xiangming Guan
Professor Emeritus of Pharmaceutical Sciences
College of Pharmacy and Allied Health Professions
Department of Pharmaceutical Sciences
Jose Gonzalez
Core Research Support Facilities Director/Professor
Division of Research and Economic Development
Department of Agronomy, Horticulture and Plant Science
Genomics Sequencing Facility
